TRAPP ing Rab18 in lipid droplets

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TRAPPing Rab18 in lipid droplets

F ar from being simple storage structures, lipid droplets (LDs) are highly dynamic organelles that are involved in several functions. Testifying to their dynamic nature, several membrane trafficking components are linked to LDs. These include ARF guanine nucleotide exchange factors (GEFs), COPI components, and several Rab GTPases. Despite the fact that almost 40 different Rab GTPases have been ...

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Rab18 localizes to lipid droplets and induces their close apposition to the endoplasmic reticulum-derived membrane.

Lipid droplets (LDs) are organelles that store neutral lipids, but their regulatory mechanism is not well understood. In the present study, we identified Rab18 as an LD component of HepG2 cells by proteomic analysis, and confirmed its localization by immunohistochemistry and western blotting. Wild-type and dominant-active Rab18 localized to LDs but the dominant-negative form did not. Endogenous...

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Rab18 Binds to Hepatitis C Virus NS5A and Promotes Interaction between Sites of Viral Replication and Lipid Droplets

Hepatitis C virus (HCV) is a single-stranded RNA virus that replicates on endoplasmic reticulum-derived membranes. HCV particle assembly is dependent on the association of core protein with cellular lipid droplets (LDs). However, it remains uncertain whether HCV assembly occurs at the LD membrane itself or at closely associated ER membranes. Furthermore, it is not known how the HCV replication ...

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ژورنال

عنوان ژورنال: The EMBO Journal

سال: 2017

ISSN: 0261-4189,1460-2075

DOI: 10.15252/embj.201696287